Expression of “Rooting” Genes iaaM and rolB Under Regulatory Promoters in Tobacco

Construction of Plant Expression Vectors Harboring WRI1 and LPAAT Genes and Its Transformation in Tobacco Plants

In oilseed crops, a number of genes involved in the production of triacylglycerol have been identified that changes in their expression have increase the seed oil content. WRI1 and LPAAT are key genes in this synthetic pathway that their overexpression can increase the oil content. In this study, the expression vectors carrying WRI1 and LPAAT genes were designed and constructed for genetic tran...

CONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS

In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...

Shared Regulatory Motifs in Promoters of Human DNA Repair Genes

Expression of related proteins and aquaporin genes in grape (Vitis vinifera L.) under salinity sress

Due to worldwide increasing of salinity, the identification of genes conferring tolerance to plants is important. The aim of this study was to investigate salinity effects on the expression of three genes-related to proteins and aquaporin in grape (Vitis vinifera L.). Based on screening study on 18 grape genotypes, H6 and Gharashani that showed lower decrease in water potential, leaf area, leaf...

Distinct regulatory modules identified in the promoters of wheat Glu-1 genes suggest different regulatory mechanisms

High molecular weight glutenin subunits of wheat are economically important seed storage proteins. They are coded by paralog pairs of the Glu-1 gene on each of the three genomes in the hexaploid wheat. Their expressions are under both temporal and spatial control. Many factors have been identified that influence the activity of Glu-1 genes, but the underlying regulatory mechanisms are still unc...